全文获取类型
收费全文 | 986篇 |
免费 | 70篇 |
国内免费 | 40篇 |
出版年
2024年 | 3篇 |
2023年 | 20篇 |
2022年 | 11篇 |
2021年 | 37篇 |
2020年 | 34篇 |
2019年 | 35篇 |
2018年 | 32篇 |
2017年 | 33篇 |
2016年 | 33篇 |
2015年 | 31篇 |
2014年 | 35篇 |
2013年 | 70篇 |
2012年 | 32篇 |
2011年 | 45篇 |
2010年 | 43篇 |
2009年 | 34篇 |
2008年 | 39篇 |
2007年 | 46篇 |
2006年 | 37篇 |
2005年 | 41篇 |
2004年 | 41篇 |
2003年 | 42篇 |
2002年 | 24篇 |
2001年 | 26篇 |
2000年 | 28篇 |
1999年 | 18篇 |
1998年 | 30篇 |
1997年 | 16篇 |
1996年 | 15篇 |
1995年 | 14篇 |
1994年 | 19篇 |
1993年 | 18篇 |
1992年 | 12篇 |
1991年 | 12篇 |
1990年 | 8篇 |
1989年 | 9篇 |
1988年 | 10篇 |
1987年 | 14篇 |
1986年 | 10篇 |
1985年 | 7篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1982年 | 7篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1976年 | 3篇 |
1974年 | 1篇 |
1973年 | 2篇 |
1972年 | 2篇 |
排序方式: 共有1096条查询结果,搜索用时 15 毫秒
111.
Tobin DJ 《Pigment cell & melanoma research》2011,24(1):75-88
Although we have made significant progress in understanding the regulation of the UVR‐exposed epidermal‐melanin unit, we know relatively little about how human hair follicle pigmentation is regulated. Progress has been hampered by gaps in our knowledge of the hair growth cycle’s controls, to which hair pigmentation appears tightly coupled. However, pigment cell researchers may have overly focused on the follicular melanocytes of the nocturnal and UVR‐shy mouse as a proxy for human epidermal melanocytes. Here, I emphasize the epidermis‐follicular melanocyte pluralism of human skin, as research models for vitiligo, alopecia areata and melanoma, personal care/cosmetics innovation. Further motivation could be in finding answers to why hair follicle and epidermal pigmentary units remain broadly distinct? Why melanomas tend to originate from epidermal rather than follicular melanocytes? Why multiple follicular melanocyte sub‐populations exist? Why follicular melanocytes are more sensitive to aging influences? In this perspective, I attempt to raise the status of the human hair follicle melanocyte and highlight some species‐specific issues involved which the general reader of the pigmentation literature (with its substantial mouse‐based data) may not fully appreciate. 相似文献
112.
Human hair melanins: what we have learned and have not learned from mouse coat color pigmentation 总被引:1,自引:0,他引:1
Hair pigmentation is one of the most conspicuous phenotypes in humans. Melanocytes produce two distinct types of melanin pigment: brown to black, indolic eumelanin and yellow to reddish brown, sulfur‐containing pheomelanin. Biochemically, the precursor tyrosine and the key enzyme tyrosinase and the tyrosinase‐related proteins are involved in eumelanogenesis, while only the additional presence of cysteine is necessary for pheomelanogenesis. Other important proteins involved in melanogenesis include P protein, MATP protein, α‐MSH, agouti signaling protein (ASIP), MC1R (the receptor for MSH and ASIP), and SLC7A11, a cystine transporter. Many studies have examined the effects of loss‐of‐function mutations of those proteins on mouse coat color pigmentation. In contrast, much less is known regarding the effects of mutations of the corresponding proteins on human hair pigmentation except for MC1R polymorphisms that lead to pheomelanogenesis. This perspective will discuss what we have/have not learned from mouse coat color pigmentation, with special emphasis on the significant roles of pH and the level of cysteine in melanosomes in controlling melanogenesis. Based on these data, a hypothesis is proposed to explain the diversity of human hair pigmentation. 相似文献
113.
The pine marten Martes martes was once considered one of Ireland's rarest carnivores, but is now recolonizing many of the areas where it was once extirpated. It is important to monitor its population expansion so that conservation management decisions can be made regarding the status of this species. In this study, we investigate the use of fur snagging devices as a method of estimating pine marten presence and abundance in lowland and upland broadleaf woodland. We investigated whether these devices reliably detected presence, and how quickly. It was found that hair traps functioned very well in detecting pine martens on all survey transects and was essential in their detection in the most isolated site. A survey length of 6 days was sufficient to detect pine marten in all survey sites, with most detections occurring within the first 4 days. The hair traps functioned equally well as scat location for detecting pine marten and had the added advantage of there being no confusion as to the origin of the sample. Live trapping was used to verify the estimates of relative abundance from hair traps. There was a trend for the number of hair samples per site and the number of times animals were trapped per site to be positively correlated, although this was not significant. Hair traps do provide a real alternative for the monitoring of pine martens in both lowland and upland broadleaf woodlands, overcoming many of the disadvantages associated with scat location. 相似文献
114.
Brice?Bathellier Friedrich?G.?BarthEmail author J?rg?T.?Albert Joseph?A.?C.?Humphrey 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2005,191(8):733-746
Arachnids and insects use long, thin hairs as motion sensors to detect signals contained in the movement of the surrounding air. These hairs often form groups with a small spacing of tens to hundreds of micrometers between them. For air oscillation frequencies of biological interest, the potential exists for viscosity-mediated coupling among hairs in a group affecting their response characteristics. Even a small diameter hair can, in principle, affect the flow field around it and the dynamics of the hairs in its neighborhood. The viscosity-mediated coupling between a pair of hairs is investigated here both experimentally and theoretically. The conditions for the existence of the coupling effect, and its magnitude as a function of relevant parameters, are determined. In the range of biologically relevant frequencies (30–300 Hz), viscous coupling between pairs of hairs is only very small in the case of the spider Cupiennius salei. Theoretical analysis points to the relatively large spacing between hairs (20 to 50 hair diameters) and the tuning of the hairs to the above-mentioned frequencies to explain the practical absence of coupling. 相似文献
115.
噪声习服对听觉损伤的保护作用机制探讨 总被引:2,自引:0,他引:2
目的:探讨噪声习服对听觉损伤的保护作用机制.方法:建立噪声习服实验动物模型.采用免疫组织化学、激光扫描共聚焦显微镜(LSCM)及图像分析等技术,定量研究噪声习服后毛细胞内纤维状肌动蛋白(F-actin)、钙调蛋白(CaM)、热休克蛋白70(HSP70)的表达及游离Ca2 浓度的变化.结果:噪声暴露后毛细胞中F-actin、CaM及HSP70的表达均呈增加趋势.与噪声损伤暴露组(H组)比较,噪声习服后损伤暴露组(CH组)中F-actin和HSP70的表达均明显增多,CaM的表达具有增加趋势.声暴露后毛细胞内游离Ca2 浓度升高,噪声损伤暴露组毛细胞内游离Ca2 浓度明显高于噪声习服组(C组)和习服后损伤暴露组.结论:噪声习服使毛细胞对于其后声刺激的保护性反应增强,毛细胞内细胞骨架系统的加强及胞内钙稳态的维持在噪声习服的保护机制中具有重要意义. 相似文献
116.
Depression of the melting temperature by moisture for alpha-form crystallites in human hair keratin 总被引:1,自引:0,他引:1
DSC thermal analysis has been carried out for human hair samples with various moisture contents to investigate the melting temperature depression behavior of alpha-form crystallites in human hair. This is achieved by adopting a novel technique using silicon oil as the thermal medium, which permits hair samples to retain a range of moisture contents in between completely dry and fully saturated. The results show that the melting temperature of the alpha-form crystallites in human hair varies with moisture content from 205 degrees C for dry hair to 155 degrees C for the hair sample with moisture content of 23%. These experimental results are particularly useful for clarification of the conceptual ambiguities associated with the molecular properties of alpha-helices and alpha-form crystallites. Furthermore, the Flory-Huggins theory was employed to determine the water-helix interaction parameter (chi = 4.5) and the alpha-form crystallinity of human hair (22%), a figure consistent with that obtained by the XRD method (21%). 相似文献
117.
In order to investigate the reduction mechanism of L-cysteine (Cys) on keratin fibers, cross-sectional samples of virgin white human hair treated with Cys were prepared. The heterogeneous reaction between Cys and keratin fibers involving the diffusion of Cys into human hair was analyzed at the molecular level using microspectrophotometry and Raman spectroscopy. The diffusion pattern of Cys into human hair showed non-Fickian type characteristics, thus indicating the free amino groups of electrostatically interacted with the anionic ions of the fiber surface. The disconnected relative concentration of -SS- groups at various depths of the hair samples with pH 9.0 was less than the Cys relative concentration, indicating that the reaction rate (the disconnection of -SS- groups) was slower than the diffusion rate of Cys into human hair. From these experiments, we concluded that the free amino groups of Cys electrostatically interacted with the anionic ions of the fiber surface, thereby decreasing the reaction rate (the disconnection of -SS- groups) of Cys at pH 9.0. 相似文献
118.
MDR-like ABC transporter AtPGP4 is involved in auxin-mediated lateral root and root hair development 总被引:17,自引:0,他引:17
Santelia D Vincenzetti V Azzarello E Bovet L Fukao Y Düchtig P Mancuso S Martinoia E Geisler M 《FEBS letters》2005,579(24):5399-5406
Previous data have suggested an involvement of MDR/PGP-like ABC transporters in transport of the plant hormone auxin and, recently, AtPGP1 has been demonstrated to catalyze the primary active export of auxin. Here we show that related isoform AtPGP4 is expressed predominantly during early root development. AtPGP4 loss-of-function plants reveal enhanced lateral root initiation and root hair lengths both known to be under the control of auxin. Further, atpgp4 plants show altered sensitivities toward auxin and the auxin transport inhibitor, NPA. Finally, mutant roots reveal elevated free auxin levels and reduced auxin transport capacities. These results together with yeast growth assays suggest a direct involvement of AtPGP4 in auxin transport processes controlling lateral root and root hair development. 相似文献
119.
120.
Kajiura Y Watanabe S Itou T Nakamura K Iida A Inoue K Yagi N Shinohara Y Amemiya Y 《Journal of structural biology》2006,155(3):438-444
The origin of the curliness of human hair was revealed by scanning microbeam small angle X-ray scattering (SAXS), based on the nanostructure of keratin fibre arrangement. Scanning microbeam SAXS patterns of single hair fibres have been measured across the fibres and the differences in the patterns between the inner and the outer sides of the curvature were successfully detected. The analysis of the equatorial and azimuthal scattering intensity profiles showed that the arrangement of the intermediate filaments was different between the inner and the outer sides of the curvature. From the analogy with Merino and Romny wool, it is suggested that different types of cortices exist in human hair. It is concluded that, regardless of the ethnic origins (African, Caucasian, and Asian), the macroscopic curl shape of the hair fibre originate from the inhomogeneity of the internal nanostructure, arising from inhomogeneous distribution of two types of cortices. 相似文献